Anti-Liver-Kidney Microsomes-1 IgG ELISA (Anti-LKM-1 IgG ELISA)
$457.00
Catalog
35-LKMHU-E01
The Anti-LKM-1 (Liver-Kidney Microsomes) (IgG) ELISA is a solid phase enzyme immunoassay employing human recombinant cytochrome p450 IID6 for the quantitative and qualitative detection of antibodies against liver-kidney microsomes (LKM) in human serum. The assay is a tool for the study of autoimmune hepatitis (AIH). Research Use Only. Not for Use in Diagnostic Procedures.
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Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
3 - 300 U/mL
Sensitivity
1.0 U/mL
Sizes
96 Wells
Sample Types
Serum
Inc Time Hour
1
Inc Time Minute
5
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen.
Antibodies, if present in the samples, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards, anti-human immunoglobulins conjugated to horseradish peroxidase
(conjugate) are incubated and react with the antigen-antibody complex of the samples in the
microplates. Unbound conjugate is washed off in the following step. Addition of TMB substrate
generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to
yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate
bound to the antigen-antibody complex and this is proportional to the initial concentration of the
respective antibodies in the sample.
