Anti-Ribosomal P IgG ELISA
$365.00
Catalog
35-RIBHU-E01
Anti-Ribosomal P (IgG) ELISA is a solid phase enzyme immunoassay employing native human ribosomal P-proteins P0, P1 and P2 isolated from eukaryotic cell line for the quantitative and qualitative detection of antibodies against ribosomal P-proteins (rib-P) in human serum. The specificity of anti-rib-P antibodies is restricted to a common antigenic determinant located on the highly conserved carboxyl-terminal portion of the three P proteins. The assay is a tool for researching diagnosis of systemic lupus erythematosus (SLE). Research Use Only. Not for Use in Diagnostic Procedures.
Minimum Order Required. Please call for details.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
3 - 300 U/mL
Sensitivity
1.0 U/mL
Sizes
96 Wells
Sample Types
Serum
Inc Time Hour
1
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
The ribosomal phosphoproteins P0 (~38 kDa), P1 (~ 19 kDa) and P2 (~17 kDa) are located within the 60S subunit of human ribosomes. In contrast to the majority of basic ribosomal proteins, P1 and P2 are acidic. The ribosomal proteins are associated to a pentamer with two P1/P2 heterodimers anchored to P0 by the amino terminal portion of P2. This pentamer is located in a highly accessible region on the stalk of the ribosome. Biochemical studies suggest that P1/P2 play a fundamental role in all three phases of ribosomal polypeptide synthesis (initiation, translocation, termination).
Research indicates that autoantibodies to ribosomal proteins are highly specific for SLE since they are not found in other autoimmune diseases or in infections. The frequency of anti-rib-P antibodies is 10-20% in randomly selected SLE subjects. Anti-rib-P antibodies are detected more frequently in lupus subjects with severe psychiatric manifestations. In addition, studies suggest that other organ involvement including renal and hepatic disease might be correlated with the presence of anti-rib-P.
