Bacterial Stool Panel I PCR Kit

$2,068.00
Catalog
86-BS1HU-100
Bacterial Stool Panel I is a multiplex real-time PCR for the direct, qualitative detection and differentiation of STEC, Salmonella spp., Campylobacter spp. and EIEC/Shigella spp. in human stool samples. Research Use Only. Not for Use in Diagnostic Procedures.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Sensitivity
≥ 10 DNA copies
Sizes
100 Tests
Sample Types
Stool
Inc Time Hour
< 1.5
Inc Time Overnight
No
Inc Time See Protocol
No
Detection
Fluorescence
The Bacterial Stool Panel I PCR Kit has been tested with the Roche LightCycler 480II and Agilent Technologies Mx3005P (with ATTO-Filter) real-time PCR instruments.  The Bacterial Stool Panel I is a multiplex real-time PCR assay for the direct, qualitative detection and differentiation of STEC, Salmonella spp., Campylobacter spp. and EIEC/Shigella spp. in human stool samples. After DNA isolation, amplification of gene fragments specific for STEC (stx1/stx2), Salmonella spp. (ttr), Campylobacter spp. (16s-rDNA) and EIEC/Shigella spp. (ipaH) occurs if present. The amplified targets are detected with hydrolysis probes, which are labeled at one end with a quencher and at the other end with a fluorescent reporter dye (fluorophore). In the presence of a target the probes hybridize to the amplicons. During the extension step the Taq-Polymerase breaks the reporterquencher proximity. The reporter emits a fluorescent signal which is detected by the optical unit of a real-time PCR instrument. The fluorescence signal increases with the amount of formed amplicons. The Bacterial Stool Panel I assay contains an Internal Control DNA (ICD) as an internal control of sample preparation procedure and to determine possible PCR inhibition.

Bacterial Stool Panel I PCR Kit

86-BS1HU-100

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