The Chicken Ovotransferrin ELISA is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ovotransferrin in biological samples of chickens. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
6.25-400 ng/mL
Sensitivity
6.25 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the ovotransferrin present in samples reacts with the anti-ovotransferrin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-ovotransferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound ovotransferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of ovotransferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of ovotransferrin in the test sample. The quantity of ovotransferrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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