Complement System C4d ELISA
$1,112.00
Catalog
13-COMPL-C4d
Complement C4d is a highly specific complement activity marker for the classical and lectin pathway
Key Features:
- Highly-specific, Reliable Measurement: The Complement System C4d assay targets a unique neoepitope only present in the C4d fragment, which enables specific assessment of complement activation and does not measure native C4 or other C4-fragments.
- Easy-to-Use: The Complement System C4d assay includes ready-to-use reagents and offers short incubation times, reducing hands-on time and simplifying workflow.
- Accelerate Drug Development: As an early activity marker in the classical and lectin pathways, C4d can help in evaluation of complement therapeutics targeting the upstream steps of the activation cascade.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
0 – 400 ng/mL
Sensitivity
4.7 ng/mL
Sizes
96 Wells
Sample Types
EDTA Plasma
Inc Time Hour
2
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
The Complement System C4d ELISA is a colorimetric sandwich assay. Samples are diluted in assay diluent and 100µL diluted sample is transferred to the microtiter wells and incubated at room temperature for 60 minutes. During this first incubation C4d in the sample is captured by the anti-C4d-Neo monoclonal antibody, pre-coated on the surface of the microtiter wells. After washing to remove unbound material, a second horseradish peroxidase (HRP) labelled monoclonal antibody, that binds to both allelic variants of C4d (A and B), is added the to the well. After incubation for 30 minutes the wells are washed again, and a substrate is added and incubated. The color development is stopped after 30 minutes, and the color is measured in a spectrophotometer. The color is directly proportional to the amount of C4d bound to the well. The amount of C4d is determined by comparison to the color development of the calibrator samples.
