The Salivary Estrone ELISA is an enzyme immunoassay for the quantitative measurement of estrone in saliva. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
10-300 pg/mL
Sensitivity
10 pg/mL
Sizes
96 Wells
Sample Types
Saliva
Inc Time Hour
1
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
The Salivary Estrone ELISA Kit is a solid-phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with streptavidin and biotinylated estrone molecules. Endogenous estrone in a sample competes with this immobilized estrone for binding sites of an HRPconjugated sheep antibody directed towards an antigenic site of estrone. After incubation, the unbound antibody conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of estrone in the sample. After the addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of estrone in the sample.
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