The Etanercept Free Anti-Drug Antibody ELISA is intended for the semi-quantitative determination of free anti-drug antibodies against Enbrel® in human serum and EDTA plasma. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Sizes
96 Wells
Sample Types
EDTA Plasma, Serum
Inc Time Hour
3
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
25
Detection
Colorimetric
This ELISA is designed for the determination of free antibodies against etanercept (e.g. ENBREL®). In a first incubation step, the free anti-etanercept antibodies from the sample are bound to the etanercept coated on the plate. To remove all unbound substances, a washing step is carried out. In a further incubation step, peroxidase-labeled antibody is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stop solution is then added. The color converts to yellow. The absorbance of the color compound is determined photometrically at 450 nm. The intensity of the color is directly proportional to the amount of bound anti-etanercept antibodies (e.g. ENBREL®) from the sample. The results are evaluated using a cut-off control.
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