Fibrinogen ELISA
$616.00
Catalog
41-FIBHU-E01
The Fibrinogen ELISA is for the determination of fibrinogen in human Serum and other Biological samples. Fibrinogen contributes to the formation of fibrin clots. As an acute phase protein, high levels of fibrinogen may be associated with cardiovascular disease (especially thrombosis). Research Use Only. Not for Use in Diagnostic Procedures.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
12.5 - 400 ng/mL
Sensitivity
12.5 ng/mL
Sizes
96 Wells
Sample Types
Biological Fluids, Plasma, Serum
Inc Time Hour
1
Inc Time Minute
40
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the
fibrinogen (FIB) present in samples reacts with the anti-fibrinogen antibodies which have been
adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by
washing, anti-fibrinogen antibodies conjugated with horseradish peroxidase (HRP), are added.
These enzyme-labeled antibodies form complexes with the previously bound fibrinogen.
Following another washing step, the enzyme bound to the immunosorbent is assayed by the
addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound
enzyme varies directly with the concentration of fibrinogen in the sample tested; thus, the
absorbance, at 450 nm, is a measure of the concentration of fibrinogen in the test sample. The
quantity of fibrinogen in the test sample can be interpolated from the standard curve constructed
from the standards and corrected for sample dilution.
