Giardia lamblia IgG ELISA Kit

The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified and inactive Giardia lamblia antigen onto the wall of microtiter well. Assay standards, controls and sample are added to wells of a microplate coated with a highly purified Giardia lamblia antigen. The Giardia lamblia antigen will bind to the antibody in the liquid standards, controls, and samples. The unbound matrices are washed away and a HRP-conjugated antibody which specifically recognizes the specific subtype of human antibody (IgG) is added for further immunoreactions. After an incubation period, an immunocomplex of “Giardia lamblia Antigen – human Anti-Giardia IgG- HRP-conjugated Anti-hIgG Antibody” is formed if the human anti-Giardia IgG is present. The unbound tracer antibody and other protein or buffer matrix are removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to the wall of each microtiter well is directly proportional to the amount of human Anti-Giardia lamblia IgG level in each sample.