The Glutamic Acid Decarboxylase ELISA (GAD ELISA) kit is an in vitro qualitative ELISA test for the detection of circulating autoantibodies to glutamic acid decarboxylase (GAD) antigen. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
0.6 - 2.99 U/mL
Sizes
96 Wells
Sample Types
Serum
Inc Time Hour
2
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
A purified GAD antigen is immobilized onto microwells. GAD specific IgG antibodies present in the serum sample are allowed to react with the antigen. The excess/unbound serum proteins are washed-off from the microwells. An enzyme (alkaline phosphatase) labeled goat antibody, specific to human IgG, is added to the GAD-antibody complex. After washing off excess unreacted enzyme conjugate from the microwells, a substrate (PNPP) is added and the color generated is measured spectrophotometrically. The intensity of the color developed directly gives the concentration of GAD autoantibodies in the test serum sample. GAD positive and negative controls serve as an internal quality control to ensure valid results.
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