The Hamster (CHO) Legumain (LGM) ELISA is quantitative immunoassay for the determination of Legumain in Chinese Hamster Ovary (CHO) cell supernatant and extract samples.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
0.31 - 20 ng/ml
Sensitivity
0.31 ng/mL
Sizes
96 Wells
Sample Types
Cell Culture
Inc Time Overnight
No
Inc Time See Protocol
No
Detection
Colorimetric
Legumain was ranked 6th out of the 30 most abundant CHO host cell proteins (HCPs) in one study of host cell proteins in batch and fed-batch CHO cultures. Legumain is a lysosomal protease that forms acidic charge variants by removing a functional group from asparagine residues (asparagine deamidation) of monoclonal antibodies, negatively impacting therapeutic antibody quality1.
1. Park, J.H. et al. (2017). Proteomic Analysis of Host Cell Protein Dynamics in the Culture Supernatants of Antibody-Producing CHO Cells. Nature: Scientific Reports. 7:44246. DOI: 10.1038/srep44246.
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