Hepatitis A Virus Antigen ELISA is for the qualitative determination of Hepatitis A Virus (HAV) Antigen in human stool and cell culture supernatant. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
Cut off Assay
Sensitivity
Cut off Assay
Sizes
96 Wells
Sample Types
Cell Culture, Stool
Inc Time Hour
4
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Detection
Colorimetric
Samples are pipetted into wells of a microplate coated with antibodies directed against Hepatitis A Virus (HAV). The HAV antigen binds to the fixed antibody and after the incubation period of two hours at 37°C the plate is washed thoroughly. Bound HAV antigen is identified with conjugate addition (monoclonal anti-HAV, peroxidase conjugated), incubated for another two hours at 37°C. Excess conjugate is removed by washing and the substrate is added. After 30 minutes incubation at room temperature the reaction is terminated by addition of stop solution. The blue color of a positive reaction turns to yellow and is measured in a microplate reader at 450 nm. The intensity of the color indicates the concentration of bound HAV antigen. A positive reaction must be confirmed by re-testing with anti-HAV neutralizing serum to discriminate false positive reactions which sometimes occur in stool.
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