IgA ELISA
$591.00
Catalog
41-IGAHU-E01
The IgA ELISA is intended for the quantitative determination of Immunoglobulin A in human serum and plasma.
- Results in only 1 hour and 10 minutes
- Ready-to-use precoated plates Research Use Only. Not for Use in Diagnostic Procedures.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
12.5-800 ng/mL
Sensitivity
12.5 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
10
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the
IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the
surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antiIgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled
antibodies form complexes with the previously bound IgA. Following another washing step, the
bound enzyme is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-
tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration
of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration
of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the
standard curve constructed from the standards and corrected for sample dilution.
