Lactoferrin ELISA
$759.00
Catalog
41-LACHU-E01
The Lactoferrin ELISA is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Lactoferrin in human biological samples. Research Use Only. Not for Use in Diagnostic Procedures.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
3.125-100 ng/mL
Sensitivity
3.125 ng/mL
Sizes
96 Wells
Sample Types
Milk, Plasma, Serum
Inc Time Hour
1
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
Lactoferrin is a multifunctional iron-binding protein with antimicrobial acitivities. It is found in milk and other mucosal secretions. Lactoferrin is known to be part of the innate defense against infection and inflammation and also has function in regulation of iron absorption.
In this assay the lactoferrin (LF) present in samples reacts with the anti-lactoferrin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antilactoferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound lactoferrin. Following another washing step, bound enzyme-conjugated antibody is assayed by the addition of a chromogenic substrate, 3,3’,5,5’- tetramethylbenzidine (TMB). The quantity of enzyme present varies directly with the concentration of LF in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of lactoferrin in the test sample. The quantity of lactoferrin in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
In this assay the lactoferrin (LF) present in samples reacts with the anti-lactoferrin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antilactoferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound lactoferrin. Following another washing step, bound enzyme-conjugated antibody is assayed by the addition of a chromogenic substrate, 3,3’,5,5’- tetramethylbenzidine (TMB). The quantity of enzyme present varies directly with the concentration of LF in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of lactoferrin in the test sample. The quantity of lactoferrin in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
