Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
12.5 - 400 ng/mL
Sensitivity
12.5 ng/mL
Sizes
96 Wells
Sample Types
Serum
Inc Time Hour
1
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay, the
ferritin present in samples reacts with the anti-ferritin antibodies which have been adsorbed to the
surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-ferritin
antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled
antibodies form complexes with the previously bound ferritin. Following another washing step,
the bound enzyme is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-
tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of
ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of
ferritin in the test sample. The quantity of ferritin in the test sample can be interpolated from the
standard curve constructed from the standards and corrected for sample dilution.
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