Mouse Transferrin ELISA is for the quantitative determination of transferrin in mouse serum and plasma. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
1.56 - 100 ng/mL
Sensitivity
1.56 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
10
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the transferrin present in samples reacts with the anti-transferrin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-transferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound transferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of transferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of transferrin in the test sample. The quantity of transferrin in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution
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