Pancreatic Elastase ELISA
$918.00
Catalog
30-PANHU-E01
The Pancreatic Elastase ELISA is intended for the quantitative determination of pancreatic elastase in human stool samples.
- Compatible with the Easy Stool Extraction Device
- Highly specific monoclonal-based assay with the broadest range on the market
FDA Class I Device. For In Vitro Diagnostic Use. , Health Canada Licensed
Additional sets of standards and controls are available for purchase using catalog number 30-PANHU-SET.
Species
Human
Regulatory Status
FDA Class I Device. For In Vitro Diagnostic Use., Health Canada Licensed
Product Distribution
Available in North America Only
Range
4.0 - 72 ng/mL
Sensitivity
1.88 ng/mL
Sizes
96 Wells
Sample Types
Stool
Inc Time Hour
1
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
15
Sample Size 2
100
Detection
Colorimetric
This ELISA is intended for the quantitative determination of pancreatic elastase in stool. In a first incubation step, the pancreatic elastase in the samples is bound to monoclonal antibodies, immobilized to the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase-labeled conjugate (mouse antipancreatic elastase) is added which specifically recognizes the bound pancreatic elastase. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB), which reacts with the peroxidase. An acidic stop solution is added to stop the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of pancreatic elastase. A dose response curve of absorbance unit (optical density, OD at 450 nm) versus concentration is generated using the values obtained from the standards.
