The Rat Adiponectin ELISA kit is a tool for the quantification of rat adiponectin in serum and plasma samples. Utilizing a dual-monoclonal antibody sandwich format, bi-level control set, and 96-well microplate comprising removable strips, a single Rat Adiponectin ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time.
Analytical sensitivity 0.08 ng/mL
Dynamic range 0.25-10 ng/mL
Low sample volume 5 µL
Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
0.25 - 10 ng/mL
Sensitivity
0.08 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
2
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
This assay is a sandwich assay using two specific and high-affinity antibodies. The adiponectin in the samples binds to the first antibody coated on the microtiter plate. In the following step, the second specific anti-adiponectin antibody binds in turn to the immobilized adiponectin. The second antibody is biotinylated and will be applied in a mixture with a Streptavidin-PeroxidaseEnzyme Conjugate. In the last substrate reaction, the color of the mixture will quantitatively depend on the adiponectin level in the samples.
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