Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
22.2 - 600 ng/mL
Sensitivity
22.2 ng/mL
Sizes
96 Wells
Sample Types
Saliva, Stool
Inc Time Hour
2
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
15
Sample Size 2
100
Detection
Colorimetric
This Enzyme-Linked Immunosorbent Assay (ELISA) is intended for the quantitative determination of secretory IgA in stool and saliva. In a first incubation step, the sIgA in the samples is bound to polyclonal antibodies (rabbit anti-human IgA), which are immobilized to the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase-labeled conjugate (mouse anti-sIgA) is added which specifically recognizes the bound secretory IgA. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB), which reacts with the peroxidase. An acidic stop solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of secretory IgA. A curve with the absorbance unit (optical density, OD) vs. concentration is generated using the results obtained from the standards. The Secretory IgA present in the samples is determined directly from this curve.
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