The 6-sulfatoxymelatonin ELISA is a quantitative immunoassay for the determination of 6-sulfatoxymelatonin (6-SMT) in urine. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
0.8 - 40 ng/mL, adjusted for dilution
Sensitivity
0.14 ng/mL
Sizes
96 Wells
Sample Types
Urine
Inc Time Hour
3
Inc Time Minute
45
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
The 6-SMT ELISA is a competitive immunoassay using a capture antibody technique (8). A polyclonal antibody specific for rabbit immunoglobulin has been coated onto the microtiter plate provided in the kit. During the first 3-hours incubation, 6-SMT present in the pre-diluted urine samples, Controls and ready to use Calibrators, respectively, compete with biotinylated 6-SMT for the binding sites of a highly specific rabbit anti-6-SMT antibody, while the formed (biotinylated) 6-SMT-antibody complexes are captured by the second antibody coated on the wells. After washing, the Enzyme Label, streptavidin conjugated to horseradish peroxidase (HRP) is added which binds during a second 30-minutes incubation step to the 6-SMT-biotin-antibody complexes captured on the coated wells. Unbound Enzyme Label is then removed by a second washing step and TMB substrate (tetramethylbenzidin) is added to the wells. In a third 30-minutes incubation step, a colored product is formed in inverse proportion to the amount of 6-SMT originally present in the sample. The color turns from blue to yellow after the addition of an acidic Stop Solution and can be measured at 450 nm. See instructions for use for references.
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