The Immundiagnostik AG assay is intended for the quantitative determination of α2 -macroglobulin in urine, serum and plasma. For research use only. Not for use in diagnostic procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
0.07 - 5 mg/L
Sensitivity
0.05 mg/L
Sizes
96 Wells
Sample Types
Plasma, Serum, Stool
Inc Time Hour
2
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
In a first incubation step, the α2 -macroglobulin in the samples is bound to polyclonal rabbit antibodies (in excess), which are immobilised to the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase-labelled anti α2 -macroglobulin antibody (POD-antibody) is added. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stop solution is then added to stop the reaction. The colour converts from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of α2 -macroglobulin in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the results obtained from the calibrators. α2 - macroglobulin, present in the subject samples, is determined directly from this curve.
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