Bacterial Stool Panel I PCR Kit

The Bacterial Stool Panel I PCR Kit has been tested with the Roche LightCycler 480II and Agilent Technologies Mx3005P (with ATTO-Filter) real-time PCR instruments.  The Bacterial Stool Panel I is a multiplex real-time PCR assay for the direct, qualitative detection and differentiation of STEC, Salmonella spp., Campylobacter spp. and EIEC/Shigella spp. in human stool samples. After DNA isolation, amplification of gene fragments specific for STEC (stx1/stx2), Salmonella spp. (ttr), Campylobacter spp. (16s-rDNA) and EIEC/Shigella spp. (ipaH) occurs if present. The amplified targets are detected with hydrolysis probes, which are labeled at one end with a quencher and at the other end with a fluorescent reporter dye (fluorophore). In the presence of a target the probes hybridize to the amplicons. During the extension step the Taq-Polymerase breaks the reporterquencher proximity. The reporter emits a fluorescent signal which is detected by the optical unit of a real-time PCR instrument. The fluorescence signal increases with the amount of formed amplicons. The Bacterial Stool Panel I assay contains an Internal Control DNA (ICD) as an internal control of sample preparation procedure and to determine possible PCR inhibition.