CeliCheck ELISA (Tissue Transglutaminase IgA/IgG ELISA)
$578.00
Catalog
35-CELHU-E01
The CeliCheck (Tissue Transglutaminase IgA/IgG) ELISA is a solid phase enzyme immunoassay for the combined quantitative and qualitative detection of IgA and IgG antibodies against neoepitopes of tissue transglutaminase (tTG) in human serum. The assay employing human recombinant tTG crosslinked with gliadin-specific peptides displays neoepitopes of tTG which ensures a significantly increased analytical sensitivity and specificity of the assay. Research Use Only. Not for Use in Diagnostic Procedures.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
3 - 300 U/mL
Sensitivity
1.0 U/mL
Sizes
96 Wells
Sample Types
Serum
Inc Time Hour
1
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Antibody’s in the sample bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample.
