Clostridium difficile PCR Kit

The Clostridium difficile PCR Kit has been tested on Roche LightCycler 480II, Agilent Technologies Mx3005P, Applied Biosystems ABI 7500, Bio-Rad CFX96, and QIAGEN Rotor-Gene Q real-time PCR instruments.  C. difficile multiplex real-time PCR assay is for the direct, qualitative detection and differentiation of C. difficile (16s-rDNA) and C. difficile toxin A (tcdA) / B (tcdB) genes in human stool samples and cultures. After DNA-isolation, amplification of the gene fragments specific for C. difficile and toxin A and B of C. difficile (if present) occurs if present. The amplified targets are detected with hydrolysis probes, which are labeled at one end with a quencher and at the other end with a fluorescent reporter dye (fluorophore). In the presence of a target, the probes hybridize to the amplicons. During the extension step, the Taq-polymerase breaks the reporter-quencher proximity. The reporter emits a fluorescence signal which is detected by the optical unit of a real-time PCR instrument. The fluorescence signal increases with the quantity of formed amplicons. The C. difficile PCR assay contains an Internal Control DNA (ICD) as an internal control of the sample preparation procedure and to determine possible PCR inhibition.