The Dopamine ELISA is an enzyme Immunoassay (ELISA) for the quantitative determination of Dopamine in plasma and urine. For Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
10 - 2000 ng/mL
Sensitivity
4.5 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Urine
Inc Time Hour
4
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
300
Sample Size 2
10
Detection
Colorimetric
Dopamine is extracted by using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.
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