EDN ELISA
$999.00
Catalog
30-EDNHU-E01
The EDN ELISA is intended for the quantitative determination of eosinophil derived neurotoxin (EPX) in human stool samples. For Research Use Only. Not for use in diagnostic procedures.
- Compatible with Easy Stool Extraction Device
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
0.25 - 16 ng/mL
Sensitivity
0.25 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum, Stool, Urine
Inc Time Hour
2
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
20
Sample Size 2
15
Detection
Colorimetric
The assay utilizes the two-site sandwich ELISA technique with two selected antibodies (monoclonal and polyclonal) that bind to human EDN.Assay standards, controls, and pre-diluted samples containing human EDN are added into wells of a microplate coated with a high affinity monoclonal anti-human EDN antibody. After the first incubation period, antibody immobilized on the wall of the microtiter wells captures human EDN in the sample. Then a peroxidase-conjugated rabbit polyclonal anti-human EDN antibody is added to each microtiter well forming a “sandwich” of capture antibody-human-EDNperoxidase-conjugate. Tetramethylbenzidine is used as a peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. Intensity of the yellow color is directly proportional to the EDN concentration. A standard curve is generated using the values obtained from the standards. EDN in the samples is determined directly from this curve.
