Feline IgE ELISA
$808.00
Catalog
41-IGEFE-E01
The Feline IgE ELISA is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the determination of IgE in feline serum and plasma samples. Research Use Only. Not for Use in Diagnostic Procedures.
Species
Feline
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
12.5-400 ng/mL
Sensitivity
12.5 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
2
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
In this assay the IgE
present in samples reacts with the anti-IgE antibodies
which have been adsorbed to the surface of
polystyrene microtitre wells. After the removal of
unbound proteins by washing, anti-IgE antibodies
conjugated with horseradish peroxidase (HRP), are
added. These enzyme-labeled antibodies form
complexes with the previously bound IgE. Following
another washing step, the enzyme bound to the
immunosorbent is assayed by the addition of a
chromogenic substcate, 3,3’,5,5’-
tetramethylbenzidine (TMB). The quantity of bound
enzyme varies directly with the concentcation of IgE
in the sample tested; thus, the absorbance, at 450
nm, is a measure of the concentcation of IgE in the
test sample. The quantity of IgE in the test sample
can be interpolated from the standard curve
constructed from the standards, and corrected for
sample dilution.
