The GABA ELISA is an enzyme immunoassay for the quantitative determination of Gamma-aminobutyric acid (GABA) in urine, plasma, serum, cell cultures and tissue homogenates.
For Research Use Only. Not for Use in Diagnostic Procedures.
After extraction and derivatization Gamma-aminobutyric acid (GABA) is quantitatively
determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is
bound to the solid phase of the microtiter plate. The derivatized analyte concentrations of the
standards, controls and samples and the solid phase bound analyte compete for a fixed
number of antiserum binding sites. When the system is in equilibrium, free antigen and free
antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is
detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is
monitored at 450 nm. Quantification of unknown samples is achieved by comparing their
absorbance with a standard curve prepared with known standards.
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