Hemoglobin / Haptoglobin Complex ELISA
$405.00
Catalog
30-HAPHU-E01
The Hemoglobin / Haptoglobin ELISA is intended for the quantitative determination of the hemoglobin/haptoglobin complex in human stool samples.
- Compatible with the Easy Stool Extraction Device
- Highly sensitive antibodies avoid the potential for both false positive and false negative results
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
0.21 - 50 µg/mL
Sensitivity
0.21 µg/mL
Sizes
96 Wells
Sample Types
Stool
Inc Time Hour
2
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
15
Sample Size 2
100
Detection
Colorimetric
This ELISA is used for quantitative determination of hemoglobin/haptoglobin complex in stool. The complex in the sample is bound to anti-haptoglobin antibodies (in excess), which are immobilized on the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, an anti-hemoglobin peroxidase-labeled antibody is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of hemoglobin/haptoglobin complex in the sample. A standard curve is generated with the values obtained from the standard (optical density vs. concentration). The presence of hemoglobin/haptoglobin complex is determined directly from this curve.
