IgD ELISA
$586.00
Catalog
41-IGDHU-E01
The IgD ELISA is intended for the quantitative measurement of IgD in human serum and plasma.
- Results in just 1 hour and 20 minutes
- Requires only 5 µL of sample Research Use Only. Not for Use in Diagnostic Procedures.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
6.25-400 ng/mL
Sensitivity
6.25 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the
IgD present in samples reacts with the anti-IgD antibodies which have been adsorbed to the
surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antiIgD antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled
antibodies form complexes with the previously bound IgD. Following another washing step, the
enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate,
3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the
concentration of IgD in the sample tested; thus, the absorbance, at 450 nm, is a measure of the
concentration of IgD in the test sample. The quantity of IgD in the test sample can be interpolated
from the standard curve constructed from the standards and corrected for sample dilution.
