The IgM ELISA is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgM in human biological samples. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
7.8-500 ng/mL
Sensitivity
7.8 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
45
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the IgM present in
samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtiter wells.
After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase
(HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following
another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic
substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration
of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test
sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the
standards, and corrected for sample dilution.
1. What percentage of your IgE allergy testing panel consists of molecular allergens rather than allergen extracts? The ALEX2 IgE allergen panel consists of 117 extracts and 183 molecular allergens. This represents the largest panel of molecular allergens on the market, including 50 unique...
Cases of food and environmental allergies have been increasing over the last decade. While conservative estimates suggest IgE-mediated allergy affects 6-8% of children and 2-3% of adults, some estimates are as high as 30%.3, 2 Symptoms can range from mild to severe and include itching, hives,...
Food sensitivity or food intolerance involves a non-allergic response to a food or food additive. In a recent on-line survey conducted in the U.S., 24% of adults aged 18 to 79 reported a food intolerance. While a food allergy is typically mediated by IgE antibodies, adverse reactions to food...
The ALPCO Glucagon ELISA is for the quantitative determination of glucagon in human EDTA plasma. This assay was developed for ease-of-use and demonstrates superior sensitivity while eliminating cross-reactivity issues present in other commercially-available assays.
ALPCO’s automated turbidimetric solution for calprotectin testing offers enhanced efficiency without compromising clinical sensitivity or specificity. Clinicians can prioritize endoscopic evaluation for the patients that need it most and quickly put IBS patients on the right treatment path.
Join our speaker from Department of Pathology , University of Utah School of Medicine, Dr. Heather Nelson, to discuss how to validate and launch a FDA-approved assay.
Energy homeostasis is a focus of research into diabetes and obesity. Read about how our assays in this area can assist both academic and industry researchers.
Complement System Solutions for Drug Development and Clinical Research Datasheet
The complement system is essential to innate immunity from a variety of diseases. ALPCO’s full range of complement system immunoassays can drive discovery.
Melatonin Products for Sleep Disorder-Related Research Datasheet
The melatonin hormone affects the circadian rhythm and sleep-wake cycle. Find out about our immunoassays and products that explore melatonin and sleep disorders.
