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Lysozyme ELISA

$605.00
Catalog
30-LYSHU-E01

The Lysozyme ELISA is intended for the quantitative determination of lysozyme in human stool samples.

Research Use Only. Not for Use in Diagnostic Procedures.
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Lysozyme ELISA Instructions for Use
Lysozyme ELISA Instructions for Use
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Lysozyme ELISA Safety Data Sheet
Lysozyme ELISA Safety Data Sheet
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Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
1.1 - 30 ng/mL
Sensitivity
1.1 ng/mL
Sizes
96 Wells
Sample Types
Stool
Inc Time Hour
2
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
15
Sample Size 2
100
Detection
Colorimetric
The assay utilizes the “sandwich” technique with two selected antibodies that recognize human lysozyme. Standards, controls, and diluted samples, which are assayed for human lysozyme, are added into the wells of a microplate coated with a high affinity anti-human lysozyme antibody. During the first incubation step, lysozyme is bound by the immobilized antibody. Then a peroxidaseconjugated anti-human lysozyme antibody is added into each microtiter well and a “sandwich” of capture antibody-human lysozyme-peroxidase conjugate is formed. Tetramethylbenzidine is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the enzymatic reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of lysozyme. A standard curve is generated using the values obtained from the standards. Lysozyme present in the samples is determined directly from this curve.

Lysozyme ELISA

30-LYSHU-E01

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