Monkey Cystatin C ELISA
$629.00
Catalog
41-CYCMK-E01
Monkey Cystatin C ELISA is for the quantitative determination of Cystatin C in monkey plasma and serum. Research Use Only. Not for Use in Diagnostic Procedures.
Species
Non-Human Primate
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
0.125 - 4 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
50
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
Cystatin C is a small cysteine proteinase inhibitor present in body fluids. Studies have shown Cystatin C levels to be directly correlated with the glomular filtration rate.
The Monkey Cystatin C ELISA is a highly sensitive two-site enzyme linked immunoassay for measuring Cystatin C in Monkey serum or plasma.
In the Monkey Cystatin C ELISA, the Cystatin C present in samples reacts with the anti-Cystatin Cantibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Cystatin C, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Cystatin C in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Cystatin C in the test sample. The quantity of Cystatin C in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
The Monkey Cystatin C ELISA is for Research Use Only.
For more information on the Monkey Cystatin C ELISA, call 800-592-5726.
The Monkey Cystatin C ELISA is a highly sensitive two-site enzyme linked immunoassay for measuring Cystatin C in Monkey serum or plasma.
In the Monkey Cystatin C ELISA, the Cystatin C present in samples reacts with the anti-Cystatin Cantibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Cystatin C, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Cystatin C in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Cystatin C in the test sample. The quantity of Cystatin C in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
The Monkey Cystatin C ELISA is for Research Use Only.
For more information on the Monkey Cystatin C ELISA, call 800-592-5726.
