Mouse C-Peptide ELISA
$630.00
Catalog
80-CPTMS-E01
The Mouse C-peptide ELISA quantifies the C-peptide protein products of mouse I and mouse II proinsulin genes. The assay utilizes a dual-monoclonal antibody sandwich format, bi-level control set, and 96-well microplate comprising removable strips. A single Mouse C-peptide ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate.
- Only 10 µL of sample is sufficient for the assay
- Superior analytical sensitivity of 7.6 pmol/L
- Broad dynamic range of 60–3,000 pmol/L
Species
Mouse
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
60 - 3000 pM
Sensitivity
7.6 pM
Sizes
96 Wells
Sample Types
Serum
Inc Time Hour
2
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
The ALPCO Mouse C-peptide ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for C-peptide. The standards, controls, and samples are added to the microplate wells with the conjugate. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. TMB Substrate is added, and the microplate is incubated a second time at room temperature on a microplate shaker at 700-900 rpm. Once the second incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of C-peptide in the sample.
