Mouse Fibrinogen ELISA
$696.00
Catalog
41-FIBMS-E01
The Mouse Fibrinogen ELISA is for the determination of fibrinogen in mouse Serum and other Biological samples. Fibrinogen contributes to the formation of fibrin clots. As an acute phase protein, high levels of fibrinogen may be associated with cardiovascular disease (especially thrombosis). Research Use Only. Not for Use in Diagnostic Procedures.
Species
Mouse
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
25 - 800 ng/mL
Sensitivity
25 ng/mL
Sizes
96 Wells
Sample Types
Citrate Plasma, Plasma, Serum
Inc Time Hour
1
Inc Time Minute
40
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the
Fibrinogen present in samples reacts with the anti-Fibrinogen antibodies which have been
adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by
washing, anti-FIB antibodies conjugated with horseradish peroxidase (HRP), are added. These
enzyme-labeled antibodies form complexes with the previously bound FIB. Following another
washing step, the bound enzyme is assayed by the addition of a chromogenic substrate, 3, 3’,5,5’-
tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration
of FIB in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration
of FIB in the test sample. The quantity of FIB in the test sample can be interpolated from the
standard curve constructed from the standards, and corrected for sample dilution.
