The Mouse IgG3 ELISA is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG3 in biological samples of mice. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
12.5-400 ng/mL
Sensitivity
12.5 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the IgG3
present in samples reacts with the anti-IgG3
antibodies which have been adsorbed to the surface
of polystyrene microtitre wells. After the removal of
unbound proteins by washing, anti-IgG3 antibodies
conjugated with horseradish peroxidase (HRP), are
added. These enzyme-labeled antibodies form
complexes with the previously bound IgG3.
Following another washing step, the enzyme bound
to the immunosorbent is assayed by the addition of a
chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine
(TMB). The quantity of bound enzyme varies directly
with the concentration of IgG3 in the sample tested;
thus, the absorbance, at 450 nm, is a measure of the
concentration of IgG3 in the test sample. The
quantity of IgG3 in the test sample can be
interpolated from the standard curve constructed
from the standards, and corrected for sample dilution.
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