The Mouse / Rat Acylated Ghrelin ELISA is for the quantitative determination of acylated ghrelin in mouse and rat plasma. For Research Use only. Not For In Vitro Diagnostic Use.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
1.95 - 250 pg/mL
Sensitivity
0.7 pg/mL
Sizes
96 Wells
Sample Types
Plasma
Inc Time Hour
3
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
100
Detection
Colorimetric
Ghrelin is present in the peripheral circulation in two forms: acylated and non-acylated. The Mouse / Rat Acylated Ghrelin ELISA specifically measures the acylated form of ghrelin. The Mouse / Rat Acylated Ghrelin ELISA is based on a double-antibody sandwich technique; the wells of the plate supplied with the kit are coated with a monoclonal antibody specific to the C-terminal part of ghrelin. This antibody will bind to any ghrelin introduced into the wells (standard or sample). The AChE - Fab` conjugate which recognizes the N-terminal part of ghrelin is also added to the wells. This allows the two antibodies to form a sandwich by binding on different parts of the acylated ghrelin. Each kit contains ghrelin AChE-Fab` conjugate, rat acylated ghrelin standard, two ghrelin quality controls, Ellman’s reagent, EIA buffer, wash buffer, Tween 20, plates pre-coated with anti-ghrelin mouse monoclonal antibody, and complete instructions.
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