Mouse / Rat CRF-HS ELISA (Mouse / Rat High Sensitivity Corticotropin Releasing Factor ELISA)
$1,386.00
Catalog
48-CRFMS-E01
The Mouse/Rat CRF-HS ELISA (Mouse/Rat High Sensitivity Corticotropin Releasing Factor ELISA) is for quantitative determination of mouse or rat CRF in mouse or rat plasma and brain extract samples. Research Use Only. Not for Use in Diagnostic Procedures.
Species
Mouse, Rat
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
0.078 - 2.5 ng/mL
Sensitivity
0.078 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Tissue Culture
Inc Time Hour
8
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
50
Detection
Colorimetric
Corticotropin releasing factor (CRF, also CRH) was initially isolated from ovine hypothalamus by Vale et
al., in 1981, and identified as a novel neuropeptide comprising 41 amino acid residues with molecular
weight 4758 (1). Later human CRF (2) and rat CRF (3) were also isolated and identified. The mouse CRF peptide is identical at amino acid level to the rat and human CRF peptides (4). CRF in anterior pituitary promotes the synthesis and secretion of ACTH, a main factor of hypothalamus-pituitary-adrenal (HPA) axis. In the rat and human, CRF distributes mainly in the hypothalamus, but it was also found in the spinal cord, stomach, spleen, duodenum, adrenal, and placenta. In addition, immunochemical evidence supported the wide
distribution of the peptide throughout the central nervous system (CNS) such as olfactory bulb, retina and central auditory system in the rat. Please see Instructions for Use for references.
