This EIA kit is used for quantitative determination of urocortin 2 in mouse plasma & serum samples. The kit is characterized by its sensitive quantification and high specificity. In addition, it has no influence by other components in samples. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
0.81 - 200 ng/mL
Sensitivity
0.82 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
20
Detection
Colorimetric
Mouse urocortin 2 standard is a highly purified synthetic product. < Specificity > The EIA kit has high specificity to mouse urocortin 2 and shows no cross reactivity with urocortin 1 (mouse, rat), urocortin 3 (mouse), ACTH (mouse, rat) or CRF (mouse, rat, human). This EIA kit for the determination of mouse urocortin 2 in samples is based on a competitive enzyme immunoassay using the combination of a highly specific antibody to mouse urocortin 2 and a biotin-avidin affinity system. Standards, samples, and labeled antigen are added to the wells of the plate coated with rabbit anti mouse urocortin 2 antibodies for a competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP) labeled streptavidin (SA) is added to form HRP labeled streptavidin-biotinylated antigen-antibody complexes on the surface of the wells. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of mouse urocortin 2 is calculated.
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