Norepinephrine ELISA
$882.00
Catalog
17-NORHU-E01.1
The Norepinephrine ELISA is an enzyme immunoassay for the quantitative determination of Noradrenaline (Norepinephrine) in plasma and urine. Research Use Only. Not for Use in Diagnostic Procedures.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
5 - 1000 ng/mL
Sensitivity
1.5 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Urine
Inc Time Hour
4
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
300
Sample Size 2
10
Detection
Colorimetric
Noradrenaline (norepinephrine) is extracted by using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically.
The competitive Norepinephrine ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.
The competitive Norepinephrine ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.
