Porcine Fibrinogen ELISA
$560.00
Catalog
41-FIBPO-E01
The Porcine Fibrinogen ELISA is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Fibrinogen in biological samples of pigs. Soluble Fibrinogen circulates in the blood and provides the material from which the insoluble fibrin clot is formed during blood coagulation. Fibrinogen is an acute phase reactant that may be a useful marker for infection and inflammation. Research Use Only. Not for Use in Diagnostic Procedures.
Species
Porcine
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
12.5-400 ng/mL
Sensitivity
12.5 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the
Fibrinogen present in samples reacts with the anti-Fibrinogen antibodies which have been adsorbed to the
surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-FIB
antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies
form complexes with the previously bound FIB. Following another washing step, the enzyme bound to the
immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine
(TMB). The quantity of bound enzyme varies directly with the concentration of FIB in the sample tested;
thus, the absorbance, at 450 nm, is a measure of the concentration of FIB in the test sample. The quantity
of FIB in the test sample can be interpolated from the standard curve constructed from the standards, and
corrected for sample dilution.
