Rabbit C-Reactive Protein ELISA (Rabbit CRP ELISA)
$840.00
Catalog
41-CRPRB-E01
The Rabbit C-Reactive Protein ELISA (Rabbit CRP ELISA) is for the determination of CRP in Serum or Plasma of rabbits. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
6.25 - 200 ng/mL
Sensitivity
5 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Minute
25
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
2
Detection
Colorimetric
In this assay, the
CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the
surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-CRP
antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled
antibodies form complexes with the previously bound CRP. Following another washing step, bound
enzyme is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine
(TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample
tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test
sample. The quantity of CRP in the test sample can be interpolated from the standard curve
constructed from the standards and corrected for sample dilution.
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