The Alpha 2-Macroglobulin ELISA is a highly sensitive two-site enzyme-linked immunoassay (ELISA) for the determination of Alpha 2-Macroglobulin in rat serum and plasma. For research use only. Not for use in diagnostic procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
6.25 - 400 ng/mL
Sensitivity
6.24 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the
alpha 2-macroglobulin (A2M) present in samples reacts with the anti-A2M antibodies which have
been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound
proteins by washing, anti-A2M antibodies conjugated with horseradish peroxidase (HRP) are
added. These enzyme-labeled antibodies form complexes with the previously bound A2M.
Following another washing step, the bound enzyme is assayed by the addition of a chromogenic
substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A2M in the sample tested; thus, the absorbance, at 450 nm, is a measure
of the concentration of alpha 2-macroglobulin in the test sample. The quantity of A2M in the test
sample can be interpolated from the standard curve constructed from the standards and corrected
for sample dilution.
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