Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
6.25 - 200 ng/mL
Sensitivity
5 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Minute
25
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
n this assay,
CRP present in samples reacts with the anti-CRP antibodies adsorbed to the surface of the
polystyrene microtiter wells. After removal of unbound proteins by washing, anti-CRP antibodies
conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies
form complexes with the previously bound CRP. Following another washing step, the enzyme
bound to the captured antibodies is assayed by the addition of the chromogenic substrate,
3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the
concentration of CRP in the sample tested, thus, the absorbance, at 450 nm, is a measure of the
concentration of CRP in the test sample. The quantity of CRP in the test sample can be
interpolated from the standard curve constructed from the standards and corrected for sample
dilution.
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