The Rat Clusterin ELISA is a competitive enzyme-linked immunosorbent assay for the quantitative determination of clusterin in rat serum, plasma, or urine.
Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
12.5-800 ng/mL
Sensitivity
12.5 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum, Urine
Inc Time Hour
1
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay,
the clusterin present in samples reacts with the anti-clusterin antibodies which have been
adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by
washing, anti-clusterin antibodies conjugated with horseradish peroxidase (HRP), are added.
These enzyme-labeled antibodies form complexes with the previously bound clusterin. Following
another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a
chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme
varies directly with the concentration of clusterin in the sample tested; thus, the absorbance, at
450 nm, is a measure of the concentration of clusterin in the test sample. The quantity of clusterin
in the test sample can be interpolated from the standard curve constructed from the standards
and corrected for sample dilution.
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