The Sex Hormone Binding Globulin ELISA (SHBG ELISA) is for the quantitative determination of SHBG by an enzyme immunoassay in human serum. For Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
3.3 - 295 nmol/L
Sensitivity
0.1 nmol/L
Sizes
96 Wells
Sample Types
Serum
Inc Time Hour
1
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
The principle of the following enzyme immunoassay follows a typical two-step capture or “sandwich” type assay. The assay makes use of two highly specific monoclonal antibodies: a monoclonal antibody specific for SHBG is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of SHBG is conjugated to horseradish peroxidase (HRP). SHBG from the sample and standards are allowed to bind to the plate, which is then washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by the addition of the stop solution. The absorbance is measured on a microtiter plate reader. The intensity of the color formed by the enzymatic reaction is directly proportional to the concentration of SHBG in the sample. A set of standards is used to plot a standard curve from which the amount of SHBG in samples and controls can be directly read.
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