Somatostatin RIA is a radioimmunoassay for the in vitro quantitative measurement of Somatostatin in human plasma. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
0 - 125 pmol/L
Sensitivity
6 pmol/L
Sizes
100 Tubes
Sample Types
Plasma
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
1
Detection
I-125
Somatostatin in plasma is extracted with Sep-pak C18 cartridges. The extracts are analysed by a competitive radioimmunoassay using an antiserum to synthetic cyclic somatostatin 14. Somatostatin in standards and samples compete with 125I-labelled somatostatin in binding to the antibodies. 125I-Tyr1-somatostatin binds in a reverse proportion to the concentration of somatostatin in standards and samples. Antibody-bound 125I-Tyr1-somatostatin is separated from the unbound fraction using the double antibody solid phase precipitation technique. The radioactivity of the precipitates is measured.
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