The TGF-β2 ELISA is an enzyme immunoassay for the quantitative measurement of TGF-β2 in serum, plasma, and cell culture supernatant. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
31.25 - 1000 pg/mL
Sensitivity
10 pg/mL
Sizes
96 Wells
Sample Types
Cell Culture, Plasma, Serum
Inc Time Hour
5
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
The TGF-β2 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwichprinciple.Prior to testing the standards and samples are diluted in assay buffer, acidified with HCl, and then neutralizedwith NaOH.Afterward, the neutralized standards and samples are added to the antibody coated (polyclonal) microtiterwells. After the first incubation, the unbound sample material is removed by washing. Then a biotinilated mouseanti TGF-β2 antibody and the Streptavidin-HRP Enzyme complex are incubated in succession. An immunoenzyme sandwich complex is formed.After incubation, the unbound conjugate is washed off. Having added the substrate solution, the intensity ofcolor developed is proportional to the concentration of TGF-β2 in the sample.
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