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Ustekinumab Drug Level ELISA

$1,620.00
Catalog
30-USLHU-E01
The Ustekinumab Drug ELISA is intended for the quantitative determination of free Stelara® concentration in human serum and EDTA plasma. Research Use Only. Not for Use in Diagnostic Procedures.
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Ustekinumab Drug Level ELISA Instructions for Use
Ustekinumab Drug Level ELISA Instructions for Use
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Ustekinumab Drug Level ELISA Safety Data Sheet
Ustekinumab Drug Level ELISA Safety Data Sheet
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Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
5.5-300 ng/mL
Sensitivity
5.5 ng/mL
Sizes
96 Wells
Sample Types
EDTA Plasma, Serum
Inc Time Hour
3
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
100
Detection
Colorimetric
This ELISA is designed to determine the quantity of free ustekinumab (therapeutic antibody against interleukins 12 and 23) in EDTA plasma or serum samples. In a first incubation step, the free ustekinumab from the sample is bound to the specific monoclonal anti-ustekinumab antibody coated on the plate. After a washing step, ustekinumab bound to the plate is detected by adding a peroxidase-labelled anti-ustekinumab antibody. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of free ustekinumab in the sample. A dose response curve of the absorbance unit (optical density, OD) versus concentration is generated, using the values obtained from the standard curve. The concentrations of free ustekinumab in the samples are determined directly from this curve

Ustekinumab Drug Level ELISA

30-USLHU-E01

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