This double antibody radioimmunoassay is designed for the quantitative in vitro diagnostic determination of immunoreactive arginine vasopressin ([Arg8]-vasopressin, anti-diuretic hormone, ADH) in EDTA plasma and urine after extraction.
Research Use Only. Not for Use in Diagnostic Procedures. Recommended for use with extration columns (12-B-AECX).
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
0.75 - 30 pg/mL
Sensitivity
1.4 pg/mL
Sizes
100 Tests
Sample Types
EDTA Plasma, Urine
Inc Time Hour
48
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
1
Detection
I-125
Immunoreactive arginine vasopressin is measured by a double-antibody radioimmunoassay using a modification of the method of Glick and Kagan (4). Extracted plasma samples, controls and calibrators are pre-incubated for 24 hours with the anti-vasopressin antibody. 125I-vasopressin competes then with vasopressin present in samples and calibrators for the same antibody binding sites. After a second 24-hours incubation, the solid phase second antibody is added to the mixture, and the antibody-bound fraction is finally precipitated and counted.
The procedure recommends a solid-phase extraction of the plasma samples and controls with reversed-phase phenylsilylsilica columns. Alternatively, an extraction of plasma samples with ethanol may also be used. However, the values using the ethanol procedure are somewhat higher due to unspecific matrix effects. See instructions for use for references.
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